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Image Search Results
Journal:
Article Title: Resonating circadian clocks enhance fitness in cyanobacteria
doi:
Figure Lengend Snippet: Growth of various cyanobacterial strains in competition with each other in batch cultures. Pairs of strains were mixed together and an aliquot was plated to form single colonies to determine the initial composition of the culture. The strains were grown for 27 days in batch culture under either a 22-h LD cycle (LD 11:11) or a 30-h LD cycle (LD 15:15), and then another aliquot of the mixed culture was plated to determine the 27-day composition of the culture. Each horizontal sequence shows the results for the pairs of strains listed on the right. The composition of the cultures was determined by observing the luminescence patterns of the colonies and counting the number of colonies on each plate exhibiting each of the strain-specific phenotypes by using a CCD camera/turntable apparatus (13). In the experiments depicted in this figure, wild type was strain AMC149. The experiment was repeated three times with essentially the same outcomes.
Article Snippet: Each mixed culture was plated onto six Petri dishes with three dishes containing spectinomycin (20 μg/μl) and the other three containing chloramphenicol (4 μg/μl), and the number of colonies on each plate was counted manually or by a
Techniques: Sequencing
Journal:
Article Title: Resonating circadian clocks enhance fitness in cyanobacteria
doi:
Figure Lengend Snippet: Characteristics of the strains used in the competition experiments. (A) The circadian phenotypes of different strains as determined with the CCD camera/turntable device described (13). All strains have a luciferase construct that reports the promoter activity of the psbAI gene (16, 22). AMC343 has almost the same phenotype as AMC149; therefore, it is not shown here. FRPs of the different strains at 30°C are approximately: 23 h (SP22), 25 h (AMC149 and AMC343), and 30 h (P28). P28R is the P28 strain that has been genetically rescued to an FRP ≈25 h. (B and C) Growth curves of the individual strains in A. On average, the doubling times for LD were one division per 16.1 h and for LL the doubling times were one division per 6.3 h.
Article Snippet: Each mixed culture was plated onto six Petri dishes with three dishes containing spectinomycin (20 μg/μl) and the other three containing chloramphenicol (4 μg/μl), and the number of colonies on each plate was counted manually or by a
Techniques: Luciferase, Construct, Activity Assay